Cloning & expression and production SAK enzyme from staphylococcus aureus and transfer in bacteria E. coli

Staphylococcus aureus is one of the most dangerous organisms that threatens life humans and animals alike. It has been found people who suffer from chronic diseases such as diabetes, blood vessels, pulmonary cancerous are more susceptible to infection, in addition ability resist antibiotics, which made fierce dangerous. This staphylococcus possessed SAK gene, acts a bio stimulant plays an important role process analyzing coagulation, it anti-plasmogen activator form equal compound human plasmin. consists chain amino acids numbering 136 acids. The study aimed isolate gene using amplification technique PCR insert into ideal vectorPET21a. And he transferred strains E.coli(BL21DE3) , stimulating bacteria by adding IPTG, purpose obtaining highest level protein expression time, then purifying affinity chromatography.